Exogenous addition of histidine reduces copper availability in the yeast Saccharomyces cerevisiae
Authors:Daisuke Watanabe, Rie Kikushima, Miho Aitoku, Akira Nishimura, Iwao Ohtsu, Ryo Nasuno, and Hiroshi Takagi
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
Keywords:
yeast Saccharomyces cerevisiae, basic amino acids, histidine cytotoxicity, copper transporter Ctr1, mitochondrial respiration.
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Conflict of interest statement:
The authors declare no conflict of interest.
Please cite this article as:
Daisuke Watanabe, Rie Kikushima, Miho Aitoku, Akira Nishimura, Iwao Ohtsu, Ryo Nasuno, and Hiroshi Takagi (2014). Exogenous addition of histidine reduces copper availability in the yeast Saccharomyces cerevisiae. Microbial Cell 1(7): 241-246.
© 2014 Watanabe et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.
Abstract:
The basic amino acid histidine inhibited yeast cell growth more severely than lysine and arginine. Overexpression of CTR1, which encodes a high-affinity copper transporter on the plasma membrane, or addition of copper to the medium alleviated this cytotoxicity. However, the intracellular level of copper ions was not decreased in the presence of excess histidine. These results indicate that histidine cytotoxicity is associated with low copper availability inside cells, not with impaired copper uptake. Furthermore, histidine did not affect cell growth under limited respiration conditions, suggesting that histidine cytotoxicity is involved in deficiency of mitochondrial copper.
doi: 10.15698/mic2014.07.154
Volume 1, pp. 241 to 246, published 07/07/2014.